期刊
DEVELOPMENT
卷 147, 期 4, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.181420
关键词
Eye; Morphogenesis; Extracellular matrix; Basement membrane; Nidogen; Neural crest
资金
- National Eye Institute/National Institutes of Health [R01 EY025378, R01 EY025780, R01 EY015128, P30 EY014800]
- University of Utah Developmental Biology Training Grant (National Institutes of Health) [T32 HD007491]
- University of Utah Genetics Training Grant (National Institutes of Health) [T32 GM007464]
Organogenesis requires precise interactions between a developing tissue and its environment. In vertebrates, the developing eye is surrounded by a complex extracellular matrix as well as multiple mesenchymal cell populations. Disruptions to either the matrix or periocular mesenchyme can cause defects in early eye development, yet in many cases the underlying mechanism is unknown. Here, using multidimensional imaging and computational analyses in zebrafish, we establish that cell movements in the developing optic cup require neural crest. Ultrastructural analysis reveals that basement membrane formation around the developing eye is also dependent on neural crest, but only specifically around the retinal pigment epithelium. Neural crest cells produce the extracellular matrix protein nidogen: impairing nidogen function disrupts eye development, and, strikingly, expression of nidogen in the absence of neural crest partially restores optic cup morphogenesis. These results demonstrate that eye formation is regulated in part by extrinsic control of extracellular matrix assembly. This article has an associated 'The people behind the papers' interview.
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