期刊
CYTOSKELETON
卷 76, 期 11-12, 页码 517-531出版社
WILEY
DOI: 10.1002/cm.21582
关键词
actin cytoskeleton; actin turnover; intracellular transport; molecular motor; myosin
类别
资金
- Army Research Office [MURI W911NF-14-1-0403]
- Human Frontier Science Program [RGY0073/2018]
- National Cancer Institute [U54 CA209992]
- National Institute of General Medical Sciences [1R01GM126256]
- National Science Foundation [ACI-1341698]
During intracellular transport, cellular cargos, such as organelles, vesicles, and proteins, are transported within cells. Intracellular transport plays an important role in diverse cellular functions. Molecular motors walking on the cytoskeleton facilitate active intracellular transport, which is more efficient than diffusion-based passive transport. Active transport driven by kinesin and dynein walking on microtubules has been studied well during recent decades. However, mechanisms of active transport occurring in disorganized actin networks via myosin motors remain elusive. To provide physiologically relevant insights, we probed motions of myosin motors in actin networks under various conditions using our well-established computational model that rigorously accounts for the mechanical and dynamical behaviors of the actin cytoskeleton. We demonstrated that myosin motions can be confined due to three different reasons in the absence of F-actin turnover. We verified mechanisms of motor stalling using in vitro reconstituted actomyosin networks. We also found that with F-actin turnover, motors consistently move for a long time without significant confinement. Our study sheds light on the importance of F-actin turnover for effective active transport in the actin cytoskeleton.
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