期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 54, 期 -, 页码 1-9出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cbpa.2019.09.004
关键词
Fluorescent reporters; Proteases; Kinases; Protein-protein interactions; In vivo imaging; Animal development
资金
- National Institute of General Medical Sciences (NIGMS) [R35 GM131766, R01 GM127664, R01 GM115399]
Dynamical features of cell signaling are the essence of living organisms. To understand animal development, it is fundamental to investigate signaling dynamics in vivo. Robust reporters are required to visualize spatial and temporal dynamics of enzyme activities and protein-protein interactions involved in signaling pathways. In this review, we summarize recent development in the design of new classes of fluorescent reporters for imaging dynamic activities of proteases, kinases, and protein-protein interactions. These reporters operate on new physical and/or chemical principles; achieve large dynamic range, high brightness, and fast kinetics; and reveal spatiotemporal dynamics of signaling that is correlated with developmental events such as embryonic morphogenesis in live animals including Drosophila and zebrafish. Therefore, many of these reporters are great tools for biological discovery and mechanistic understanding of animal development and disease progression.
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