期刊
CROP PROTECTION
卷 126, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2019.104915
关键词
Bacillus thuringiensis; Spodoptera frugiperda; F-2 screen; Resistance; Vip3Aa
类别
Fall armyworm, Spodoptera frugiperda, is a major target pest of pyramided Bt (Bacillus thuringiensis) corn and cotton in the U.S. In this study, we used a well-established F-2 screening method to detect resistance alleles to Vip3Aa51 protein in field populations of S. frugiperda in Texas. In 2018, a total of 95 F-2 two-parent families of S. frugiperda were established using single-pair mating of field individuals collected from two sorghum fields in two locations in Texas. For each F-2 family, 128 neonates were screened on a diagnostic concentration of 3.0 mu g/cm(2) Vip3Aa51 protein using diet-overlay bioassays. The results showed that two out of 95 two-parent families possessed a major resistance allele against Vip3Aa51 protein. The estimated frequency of major resistance alleles against Vip3Aa51 in S. frugiperda in Texas was 0.0078 with a 95% CI of 0.0016-0.0188. The Vip3Aa51-resistant strain (TX-RR) derived from the F-2 screening showed a high level of resistance to Vip3Aa51 protein, with a resistance ratio of >395.0-fold relative to a susceptible population (SS). Information generated from this study should be useful in studying mechanisms of Vip3Aa resistance in S. frugiperda, resistance monitoring, and resistance management of the Vip3Aa technology.
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