期刊
COLLOIDS AND SURFACES B-BIOINTERFACES
卷 185, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.colsurfb.2019.110586
关键词
Titanium; Alpha-helical antimicrobial peptide; Biofunctionalization; Staphylococcus epidermidis; Osteoblasts; Osteoblast-bacteria co-culture
资金
- departmental research funds (Department of Medicine, University of Udine, Italy)
- Generalitat de Catalunya [2017SGR-1165]
- Ministry of Science and Innovation, Spain [MAT2015-67183-R]
Bacterial infection of orthopaedic implants, often caused by Staphylococcus species, may ultimately lead to implant failure. The development of infection-resistant, osteoblast-compatible biomaterials could represent an effective strategy to prevent bacterial colonization of implants, reducing the need for antibiotics. In this study, the widely used biomaterial titanium was functionalized with BMAP27(1-18), an alpha-helical cathelicidin antimicrobial peptide that retains potent staphylocidal activity when immobilized on agarose beads. A derivative bearing a short spacer with a free thiol at the N-terminus was coupled to silanized titanium disks via thiol-maleimide chemistry. Tethering was successful, as assessed by Contact angle, Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), and X-ray Photoelectron Spectroscopy (XPS), with an average surface mass density of 456 ng/cm(2) and a layer thickness of 3 nm. The functionalized titanium displayed antimicrobial properties against a reference strain of Staphylococcus epidermidis with well-known biofilm forming capability. Reduction of bacterial counts and morphological alterations of adhering bacteria, upon 2 h incubation, indicate a rapid contact-killing effect. The immobilized peptide was not toxic to osteoblasts, which adhered and spread better on functionalized titanium when co-cultured with bacteria, compared to non-coated surfaces. Results suggest that functionalization of titanium with BMAP27(1-18) could be promising for prevention of bacterial colonization in bone graft applications.
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