期刊
CLINICS IN PLASTIC SURGERY
卷 47, 期 1, 页码 99-+出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.cps.2019.08.012
关键词
Stem cells; Adipose-derived stem cells; Stem cell-enriched lipotransfer; Fat graft; Fat transplantation; Stromal vascular fraction
类别
Autologous fat graft has limitations, especially long-term unpredictability of volume maintenance. The mechanical enrichment of fat graft with adipose-derived stem cells (ADSCs) could guarantee the survival of fat grafts. After decantation, washing, and centrifugation of lipoaspirate, the authors carried out histochemical analysis and flow cytometry to determine the best layers for preparing ADSC-enriched fat. After centrifugation, the stromal vascular fraction (SVF) was separated by mechanical dissociation and mixed with another layer of intact adipocytes, which was injected into patients. All patients showed volumetric improvement after a single lipotransfer section, without overcorrection. The method is safe, has low cost, and is easily reproducible.
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