期刊
BIORESOURCE TECHNOLOGY
卷 185, 期 -, 页码 269-275出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.03.011
关键词
Adaptive laboratory evolution; High CO2 concentration tolerance; Chlorella sp.; CO2 fixation
资金
- Shanghai Pujiang Project [12PJ1408000]
- Science and Technology Commission of Shanghai Municipality Program [13DZ1205000, 14DZ1203800]
CO2 capture by microalgae is a promising method to reduce greenhouse gas emissions. It is critical to construct a highly efficient way to obtain a microalgal strain tolerant to high CO2 concentrations with high CO2 fixation capability. In this study, two evolved Chlorella sp. strains, AE10 and AE20 were obtained after 31 cycles of adaptive laboratory evolution (ALE) under 10% and 20% CO2, respectively. Both of them grew rapidly in 30% CO2 and the maximal biomass concentration of AE10 was 3.68 +/- 0.08 g/L, which was 1.22 and 2.94 times to those of AE20 and original strain, respectively. The chlorophyll contents of AE10 and AE20 were significantly higher than those of the original one under 1-30% CO2. The influences of ALE process on biochemical compositions of Chlorella cells were also investigated. This study proved that ALE was an effective approach to improve high CO2 tolerance of Chlorella sp. (C) 2015 Elsevier Ltd. All rights reserved.
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