期刊
BIORESOURCE TECHNOLOGY
卷 189, 期 -, 页码 15-22出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.03.141
关键词
Hemicellulose; Hydrolysate; Ethanol; Furfural; Inhibitors
资金
- U.S. Department of Agriculture [2011-10006-30358, 2012-67009-19596]
- US Department of Energy's Office of International Affairs [DE-PI0000031]
- BASF
- Florida Department of Agriculture and Consumer Services
- NIFA [578507, 2012-67009-19596] Funding Source: Federal RePORTER
Inhibitory side products from dilute acid pretreatment is a major challenge for conversion of lignocellulose into ethanol. Six strategies to detoxify sugarcane hydrolysates were investigated alone, and in combinations (vacuum evaporation of volatiles, high pH treatment with ammonia, laccase, bisulfite, microaeration, and inoculum size). High pH was the most beneficial single treatment, increasing the minimum inhibitory concentration (measured by ethanol production) from 15% (control) to 70% hydrolysate. Combining treatments provided incremental improvements, consistent with different modes of action and multiple inhibitory compounds. Screening toxicity using tube cultures proved to be an excellent predictor of relative performance in pH-controlled fermenters. A combination of treatments (vacuum evaporation, laccase, high pH, bisulfite, microaeration) completely eliminated all inhibitory activity present in hydrolysate. With this combination, fermentation of hemicellulose sugars (90% hydrolysate) to ethanol was complete within 48 h, identical to the fermentation of laboratory xylose (50 g/L) in AM1 mineral salts medium (without hydrolysate). (C) 2015 Elsevier Ltd. All rights reserved.
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