期刊
CHEMSUSCHEM
卷 13, 期 3, 页码 564-573出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cssc.201902928
关键词
1; 3-propanediol; bacteria; electrochemical conversion; electrofermentation; metabolic shift
资金
- Mid-Career Researcher Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning, Korea [NRF-2018R1A2B6005460]
- C1 Gas Refinery Programs through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning, Korea [NRF-2018M3D3A1A01055756, NRF-2015M3D3A1A01064929]
- National Research Foundation of Korea [2018M3D3A1A01055756] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Electrofermentation actively regulates the bacterial redox state, which is essential for bioconversion and has been highlighted as an effective method for further improvements of the productivity of either reduced or oxidized platform chemicals. 1,3-Propanediol (1,3-PDO) is an industrial value-added chemical that can be produced from glycerol fermentation. The bioconversion of 1,3-PDO from glycerol requires additional reducing energy under anoxic conditions. The cathode-based conversion of glycerol to 1,3-PDO with various electron shuttles (2-hydroxy-1,4-naphthoquinone, neutral red, and hydroquinone) using Klebsiella pneumoniae L17 was investigated. The externally poised potential of -0.9 V vs. Ag/AgCl to the cathode increased 1,3-PDO (35.5 +/- 3.1 mm) production if 100 mu m neutral red was used compared with non-bioelectrochemical system fermentation (23.7 +/- 2.4 mm). Stoichiometric metabolic flux and transcriptional analysis indicated a shift in the carbon flux toward the glycerol reductive pathway. The homologous overexpression of glycerol dehydratase (DhaB) and 1,3-PDO oxidoreductase (DhaT) enzymes synergistically enhanced 1,3-PDO conversion (39.3 +/- 0.8 mm) under cathode-driven fermentation. Interestingly, a small current uptake (0.23 mmol of electrons) caused significant metabolic flux changes with a concomitant increase in 1,3-PDO production. This suggests that both an increase in 1,3-PDO production and regulation of the cellular metabolic pathway are feasible by electrode-driven control in cathodic electrofermentation.
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