期刊
BONE
卷 84, 期 -, 页码 69-77出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2015.12.010
关键词
Heterotopic ossification; Mesenchymal progenitor; Alpha smooth muscle actin; Satellite cell; Osteogenesis
资金
- National Institute of Arthritis and Musculoskeletal and Skin Diseases of the National Institutes of Health [AR055607]
- Connecticut Stem Cell Research Initiative [14-SCA-UCHC-02]
Heterotopic ossification (HO) is a pathological process where bone forms in connective tissues such as skeletal muscle. Previous studies have suggested that muscle-resident non-myogenic mesenchymal progenitors are the likely source of osteoblasts and chondrocytes in HO. However, the previously identified markers of muscle resident osteoprogenitors label up to half the osteoblasts within heterotopic lesions, suggesting other cell populations are involved. We have identified alpha smooth muscle actin (alpha SMA) as a marker of osteoprogenitor cells in bone and periodontium, and of osteo-chondro progenitors in the periosteum during fracture healing. We therefore utilized a lineage tracing approach to evaluate whether alpha SMACreERT2 identifies osteoprogenitors in the muscle. We show that in the muscle, aSMACreERT2 labels both perivascular cells, and satellite cells. alpha SMACre-labeled cells undergo osteogenic differentiation in vitro and form osteoblasts and chondrocytes in BMP2-induced HO in vivo. In contrast, Pax7CreERT2-labeled muscle satellite cells were restricted to myogenic differentiation in vitro, and rarely contributed to HO in vivo. Our data indicate that aSMACreERT2 labels a large proportion of osteoprogenitors in skeletal muscle, and therefore represents another marker of muscle resident cells with osteogenic potential under HO-inducing stimulus. In contrast, muscle satellite cells make minimal contribution to bone formation in vivo. (C) 2015 Elsevier Inc. All rights reserved.
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