期刊
BIORESOURCE TECHNOLOGY
卷 181, 期 -, 页码 231-237出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.01.040
关键词
Nannochloropsis salina; Conditioned medium; Colonization; Transformation efficiency; Fatty acid methyl ester (FAME)
资金
- Advanced Biomass R&D Center (ABC) of Korea - Ministry of Science, ICT and Future Planning [ABC-2010-0029728, 2011-0031350]
The oleaginous microalga Nannochloropsis sp. has been spotlighted as a promising candidate in genetic engineering research for biodiesel production. However, one of the major bottlenecks in the genetic manipulation against Nannochloropsis sp. is low transformation efficiency. Based on the idea that they grow rapidly in broth culture, the effect of conditioned medium on colonization and transformation efficiency of Nannochloropsis salina was investigated. Cells grown on agar plates with 20-40% conditioned medium produced colonies that were approximately 2.3-fold larger than cells grown without conditioned medium. More importantly, the transformation efficiency was about 2-fold greater on plates with 30% conditioned medium relative to those without conditioned medium. In addition, FAME productivity in liquid cultures with 100% conditioned medium increased up to 20% compared with cultures of control medium. These results suggest that conditioned medium can be applied for efficient transformation and cost-effective cultivation of N. salina for biodiesel production. (C) 2015 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据