期刊
CHEMBIOCHEM
卷 21, 期 12, 页码 1717-1722出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900784
关键词
biosynthesis; gene expression; nitrogen fixation; non-diazotrophic hosts; protein engineering
资金
- National Key Research and Development Program of China [2019YFA0904700]
- Innovative Project of SKLAB [2019SKLAB1-1]
Nitrogen is one of the most important nutrients for plant growth. To enhance crop productivity, chemical nitrogen fertilizer is commonly applied in agriculture. Biological nitrogen fixation, the conversion of atmospheric N-2 to NH3, is an important source of nitrogen input in agriculture and represents a promising substitute for chemical nitrogen fertilizers. However, nitrogen fixation is only sporadically distributed within bacteria and archaea (diazotrophs). Thus, many biologists hope to reconstitute a nitrogenase biosynthetic pathway in a eukaryotic host, with the final aim of developing N-2-fixing cereal crops. With the advent of synthetic biology and a deep understanding of the fundamental genetic determinants necessary to sustain nitrogen fixation in bacteria, much progress has been made toward this goal. Transfer of native and refactored nif (nitrogen fixation) genes to non-diazotrophs has been attempted in model bacteria, yeast, and plants. Specifically, nif genes from Klebsiella oxytoca, Azotobacter vinelandii, and Paenibacillus polymyxa have been successfully transferred and expressed in Escherichia coli, Saccharomyces cerevisiae, and even in the tobacco plant. These advances have laid the groundwork to enable cereal crops to fix nitrogen themselves to sustain their growth and yield.
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