期刊
CHEMBIOCHEM
卷 21, 期 9, 页码 1364-1371出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900649
关键词
adenosine kinase; enzymes; methylation; psilocybin; S-adenosylhomocysteine hydrolase
资金
- Deutsche Forschungsgemeinschaft (DFG) [HO2515/7-1]
- Usona Institute (Madison, WI, USA)
- DFG Collaborative Research Center ChemBioSys 1127 [B05]
Psychotropic Psilocybe mushrooms biosynthesize their principal natural product psilocybin in five steps, among them a phosphotransfer and two methyltransfer reactions, which consume one equivalent of 5 '-adenosine triphosphate (ATP) and two equivalents of S-adenosyl-l-methionine (SAM). This short but co-substrate-intensive pathway requires nucleoside cofactor salvage to maintain high psilocybin production rates. We characterized the adenosine kinase (AdoK) and S-adenosyl-l-homocysteine (SAH) hydrolase (SahH) of Psilocybe cubensis. Both enzymes are directly or indirectly involved in regenerating SAM. qRT-PCR expression analysis revealed an induced expression of the genes in the fungal primordia and carpophores. A one-pot in vitro reaction with the N-methyltransferase PsiM of the psilocybin pathway demonstrates a concerted action with SahH to facilitate biosynthesis by removal of accumulating SAH.
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