期刊
CHEMBIOCHEM
卷 21, 期 9, 页码 1293-1297出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900664
关键词
biosensors; DNAzymes; metal ions; rational evolution; RNA cleavage
资金
- National Key R&D Program of China [2018YFC0311106]
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Collaborative Innovation Center for Jiangsu Marine Bio-Industry Technology
- NAU Doctoral overseas Academic Fund
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
In 1994, the first DNAzyme named GR5 was reported, which specifically requires Pb2+ for its RNA cleavage activity. Three years later, the 8-17 DNAzyme was isolated. The 8-17 DNAzyme and the related 17E DNAzyme are also most active with Pb2+, although other divalent metals can work as well. GR5 and 17E have the same substrate sequence, and their catalytic loops in the enzyme strands also have a few similar and conserved nucleotides. Considering these, we hypothesized that 17E might be a special form of GR5. To test this hypothesis, we performed systematic rational evolution experiments to gradually mutate GR5 toward 17E. By using the activity ratio in the presence of Pb2+ and Mg2+ for defining these two DNAzymes, the critical nucleotide was identified to be T-12 in 17E for metal specificity. In addition, G(9) in GR5 is a position not found in most 17E or 8-17 DNAzymes, and G(9) needs to be added to rescue GR5 activity if T-12 becomes a cytosine. This study highlights the links between these two classic and widely used DNAzymes, and offers new insight into the sequence-activity relationship related to metal selectivity.
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