期刊
CHEMBIOCHEM
卷 21, 期 6, 页码 785-788出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.201900489
关键词
enzyme catalysis; high-resolution melting; kinetics; ligases; quantitative analysis
资金
- National Natural Science Foundation of China [31571937]
- Shandong Provincial Natural Science Foundation of China [ZR2019BC096]
- Fundamental Research Funds for the Central Universities [201812009]
High-resolution melting (HRM) analysis has been improved and applied for the first time to quantitative analysis of enzymatic reactions. By using the relative ratios of peak intensities of substrates and products, the quantitativity of conventional HRM analysis has been improved to allow detailed kinetic analysis. As an example, the ligation of sticky ends through the action of T4 DNA ligase has been kinetically analyzed, with comprehensive data on substrate specificity and other properties having been obtained. For the first time, the kinetic parameters (k(obs) and apparent K-m) of sticky-end ligation were obtained for both fully matched and mismatched sticky ends. The effect of ATP concentration on sticky-end ligation was also investigated. The improved HRM method should also be applicable to versatile DNA-transforming enzymes, because the only requirement is that the products have T-m values different enough from the substrates.
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