期刊
CELLULAR IMMUNOLOGY
卷 349, 期 -, 页码 -出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cellimm.2020.104042
关键词
Target nanoparticles; Interleukin-12; Hepatocellular carcinoma; Cytotoxic anti-tumor T lymphocytes; Cancer immunotherapy
资金
- Fujian Provincial Health Technology Project [2016-CX-11]
- Open research fund of key laboratory of ministry of education for gastrointestinal cancer, Fujian Medical University [FMUGIC-201702]
Cytotoxic T lymphocytes (CTLs) play a major role in cancer immunotherapy. A potent tumor immunotherapy may not only require activation of anti-tumor effector cells but also rely on the use of cytokines to create a controlled environment for the development of anti-tumor T cells. In this study, we fabricated a dual-target immunonanoparticle, e.g. poly(D,L-lactide-co-glycolide) nanoparticle, by loading Interleukin-12 (IL-12) and modifying with CD8 and Glypican-3 antibodies on the surface. Our results demonstrate that the fabricated targeting immunonanoparticles bind specifically to the two target cells of interest, i.e. CD8 + T cells and HepG-2 cells via the antibody-antigen interactions and form T cell-HepG-2 cell clusters, which enhances the cytotoxicity of T cells. IL-12-containing dual-target immunonanoparticles delivered IL-12 specifically to CD8 + T cells, and favored the expansion, activation and cytotoxic activity of CD8 + T lymphocytes. These results suggest that dual-target IL-12-encapsulated nanoparticles are a promising platform for cancer immunotherapy.
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