期刊
CELL STEM CELL
卷 25, 期 6, 页码 768-+出版社
CELL PRESS
DOI: 10.1016/j.stem.2019.10.006
关键词
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资金
- Max Planck Society
- University of Munster
- Deutsche Forschungsgemeinschaft cluster of excellence Cells in Motion''
- European Research Council [AdG 339409, AdG 786672]
- Chinese Academy of Science
- Christiane Nusslein-Volhard Stiftung
Radiotherapy and chemotherapy disrupt bone vasculature, but the underlying causes and mechanisms enabling vessel regeneration after bone marrow (BM) transplantation remain poorly understood. Here, we show that loss of hematopoietic cells per se, in response to irradiation and other treatments, triggers vessel dilation, permeability, and endothelial cell (EC) proliferation. We further identify a small subpopulation of Apelin-expressing (Apln(+)) ECs, representing 0.003% of BM cells, that is critical for physiological homeostasis and transplant-induced BM regeneration. Genetic ablation of Apln ECs or Apin-CreER-mediated deletion of Kitl and Vegfr2 disrupt hematopoietic stem cell (HSC) maintenance and contributions to regeneration. Consistently, the fraction of Apln(+) ECs increases substantially after irradiation and promotes normalization of the bone vasculature in response to VEGF-A, which is provided by transplanted hematopoietic stem and progenitor cells (HSPCs). Together, these findings reveal critical functional roles for HSPCs in maintaining vascular integrity and for Apln(+) ECs in hematopoiesis, suggesting potential targets for improving BM transplantation.
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