期刊
CELL METABOLISM
卷 31, 期 3, 页码 642-+出版社
CELL PRESS
DOI: 10.1016/j.cmet.2020.02.003
关键词
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资金
- Russian Science Foundation, Russia [17-14-01086, 19-14-00108]
- Deutsche Forschungsgemeinschaft (DFG), Germany [IRTG1816, SFB1190, SFB1027]
- National Institutes of Health, United States [P30 DK057521, R21 AG063073]
- Brigham and Women's Hospital Health and Technology Innovation Award, United States
- Austrian Science Foundation, Austria, grant FWF [J4113]
- Vlaams Instituut voor Biotechnologie (VIB), Belgium
- Strategic Research Programme of the VUB, Belgium [SRP34]
- Research Foundation-Flanders, Belgium, Excellence of Science project [30829584]
- Government of Flanders IWT PhD fellowship, Belgium
- Ministry of Science and Higher Education of the Russian Federation, Russia [075-15-2019-1789]
- Russian Foundation for Basic Research, Russia [19-315-80018]
- Russian Science Foundation [19-14-00108] Funding Source: Russian Science Foundation
- Austrian Science Fund (FWF) [J4113] Funding Source: Austrian Science Fund (FWF)
Hydrogen peroxide (H2O2) is a key redox intermediate generated within cells. Existing probes for H2O2 have not solved the problem of detection of the ultra-low concentrations of the oxidant: these reporters are not sensitive enough, or pH-dependent, or insufficiently bright, or not functional in mammalian cells, or have poor dynamic range. Here we present HyPer7, the first bright, pH-stable, ultrafast, and ultrasensitive ratiometric H2O2 probe. HyPer7 is fully functional in mammalian cells and in other higher eukaryotes. The probe consists of a circularly permuted GFP integrated into the ultrasensitive OxyR domain from Neisseria meningitidis. Using HyPer7, we were able to uncover the details of H2O2 diffusion from the mitochondrial matrix, to find a functional output of H2O2 gradients in polarized cells, and to prove the existence of H2O2 gradients in wounded tissue in vivo. Overall, HyPer7 is a probe of choice for real-time H2O2 imaging in various biological contexts.
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