期刊
BIORESOURCE TECHNOLOGY
卷 176, 期 -, 页码 156-162出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.10.153
关键词
Glycerol; Ethanol tolerance; Ribosome engineering; Klebsiella variicola; Ethanol production
资金
- Environment Research and Technology Development Fund (ERTDF) from the Ministry of the Environment, Government of Japan [3K123007]
To improve the ethanol tolerance of the Klebsiella variicola strain TB-83, we obtained the streptomycin-resistant, ethanol-tolerant mutant strain TB-83D by a ribosome engineering approach. Strain TB-83D was able to grow in the presence of 7% (v/v) ethanol and it showed higher ethanol production than strain TB-83. Examination of various culture conditions revealed that yeast extract was essential for ethanol production and bacterial growth. In addition, ethanol production was elevated to 32 g/L by the addition of yeast extract; however, ethanol production was inhibited by formate accumulation. With regard to cost reduction, the use of corn steep liquor (CSL) markedly decreased the formate concentration, and 34 g/L ethanol was produced by combining yeast extract with CSL. Our study is the first to improve ethanol tolerance and productivity by a ribosome engineering approach, and we found that strain TB-83D is effective for ethanol production from glycerol. (C) 2014 Elsevier Ltd. All rights reserved.
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