期刊
CELL
卷 179, 期 7, 页码 1566-+出版社
CELL PRESS
DOI: 10.1016/j.cell.2019.11.022
关键词
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资金
- National Key R&D Program of China [2017YFA0504400]
- Chinese Academy of Sciences [XDB19010203]
- National Natural Science Foundation of China [31830109, 31821004, 91940305, 91640201]
- Shanghai Municipal Science and Technology Major Project [17JC1420100, 19JC1410200, 2017SHZDZX01]
- Foundation of Key Laboratory of Gene Engineering of the Ministry of Education
- SA-SIBS Scholarship Program
- China Postdoctoral Science Foundation [2016M600335]
- NIH [GM052872, HG004659]
Spermiogenesis is a highly orchestrated developmental process during which chromatin condensation decouples transcription from translation. Spermiogenic mRNAs are transcribed earlier and stored in a translationally inert state until needed for translation; however, it remains largely unclear how such repressed mRNAs become activated during spermiogenesis. We previously reported that the MIWI/piRNA machinery is responsible for mRNA elimination during late spermiogenesis in preparation for spermatozoa production. Here we unexpectedly discover that the same machinery is also responsible for activating translation of a subset of spermiogenic mRNAs to coordinate with morphological transformation into spermatozoa. Such action requires specific base-pairing interactions of piRNAs with target mRNAs in their 30 UTRs, which activates translation through coupling with cis-acting AU-rich elements to nucleate the formation of a MIWI/piRNA/eIF3f/HuR super-complex in a developmental stage-specific manner. These findings reveal a critical role of the piRNA system in translation activation, which we show is functionally required for spermatid development.
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