Structural characterization and antioxidant activity of a new polysaccharide from Bletilla striata fibrous roots

A new polysaccharide (pFSP) was first isolated from the originally discarded fibrous roots part of Chinese traditional herb, Bletilla striata. pFSP was composed of D-glucose, D-galactose and D-mannose in a molar ratio of 1: 2.03: 3.45 with molecular weight of 9.1 x 10(4)Da. It could effectively scavenge DPPH and superoxide radicals with inhibition rate of 64.47% and 72.27% at 5.0 mg/ml, higher than that of polysaccharide from Bletilla striata tuber. Structural investigations of the periodate oxidation studies and Smith-degradation as well as the FT-IR spectroscopy were performed, and combined with 1D and 2D NMR spectroscopy, the repeating unit of pFSP contained (1 -> 4)-linked-alpha-D-Glcp, (1 -> 4) linked-beta -D Manp and (1 -> 3,6) linked-beta-D-Manp units, together with the branches of (1 -> 6)-linked-beta-D-Galp and terminated with (1 ->) linked-beta-D-Manp residue.