期刊
CARBOHYDRATE POLYMERS
卷 226, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115330
关键词
Starch; Structure; Function; Asymmetrical flow field-flow fractionation
资金
- Nature Science Foundation of Hebei Province [B2016201002]
- Key Project of Hebei Education Department [ZD2019009]
Starch is one of the most abundant, renewable, non-toxic, and biodegradable polysaccharides, mainly comprising amylose and amylopectin. The physicochemical properties and structure of starch affect the quality of starch-based products. Therefore, the accurate characterization of the physicochemical properties and structure of starch is important to obtain products that meet specific functions. Recently, asymmetrical flow field-flow fractionation coupled with multi-angle light scattering (MALS) and differential refraction detectors (dRI) (AF4-MALS-dRI) has attracted great attention in the separation and characterization of starch. In this review, the structure and functions of starch characterized by AF4-MALS-dRI are summarized. The principle and important characterization parameters of AF4 are introduced. The starch structure-function relationship is deeply investigated owing to utilization of AF4-MALS-dRI. Besides separation of starch, AF4-MALS-dRI can provide more information about starch sample, such as radius of gyration (R-g), hydrodynamic radius (R-h), molar mass (M-w), apparent density (rho), and conformation (R-g/R-h) of starch molecules. These parameters can be further used to investigate the structure-function relationship of starch.
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