期刊
CARBOHYDRATE POLYMERS
卷 232, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115802
关键词
Oxidative stress; Protein carbonyl groups; Human blood serum; Hydrazide-carbonyl click chemistry; Protein gel electrophoresis; Carbonyl scavengers
资金
- National Key R&D Program of China [2018YFC1105900]
- Guangxi Science and Technology Base and Talent Special Project [GuikeAD17129012]
- local Science and Technology Development Project leading by the central government (the three-D printing and digital medical platform) [GuikeZY18164004]
A series of biocompatible and non- toxic polysaccharide molecules have been successfully fabricated and explored their potential application for scavenging the carbonyl species in vitro. These macromolecules were dextrans with different hydrazide substitution ratios determined by TNBS assay, NMR and FTIR characterization. The colorimetric assay had demonstrated that these macromolecules could effectively scavenge acrolein, oxidized bovine serum albumin (BSA) in buffer solutions as well as carbonyl proteins from serum. The scavengers could achieve twice more scavenging effects for modified dextrans with high molecular weight (Mw=100,000) than those of low ones (Mw=40,000) with the same substitution ratio. Protein gel electrophoresis confirmed that the formation of the complex between carbonyls and modified dextrans resulted in appearance of slower bands. It also revealed that such macromolecules could protect cultured cells against the toxicity of acrolein or its derivatives. The proposed macromolecules indicated a very promising capability as scavengers for oxidative stress plus its derivatives without side effects.
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