Juniperus pingii var. wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity

A water-soluble acidic polysaccharide, XB-PS3, was isolated from the twigs of Juniperus pingii var. Wilsonii with a molecular weight of 86.04 kDa. By means of monosaccharide composition analysis, methylation, 2D NMR spectroscopy and UPLC-MS analysis, we concluded that XB-PS3 had a backbone composed of -> 2,4)-alpha-Manp-(1 -> and -> 4)-alpha-GalpA-(1 -> (60 % esterified), with an araban branch attached to O-2 of -> 2,4)-alpha-Manp-(1 -> The possible repeating units were further validated by oligosaccharide analysis and partial acid hydrolysis. XB-PS3 exhibited potent anticomplement activity with CH50 value of 117.23 +/- 18.74 mu g/mL and interacted with C3, C4, C5 and C9 in the complement activation cascade. However, the anticomplement activity was significantly weakened when the galacturonic acids were reduced (CH50 : 268.55 +/- 16.82 mu g/mL) or the branches were removed by partial hydrolysis (CH50 : 197.76 +/- 21.81 mu g/mL), indicating the important role of uronic acids and branch structure in the polysaccharide's anticomplement activity.