期刊
CARBOHYDRATE POLYMERS
卷 229, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115497
关键词
Ulva clathrate; Sulfated polysaccharide; Structure; Ulvan lyase
资金
- National key research and development [program2018YFC0311203]
- Key Research and Development Program of Shandong2018GHY115032
Rhamnan-rich sulfated polysaccharides extracted from green algae (ulvan) constitute potentially useful natural materials for drug development. However, the characterization of their complex structures poses a challenge for their application. In this study, the structure of ulvan extracted from Ulva clathrata was analyzed with the assistance of an ulvan lyase belonging to the PL25 family. According to mass spectrometry and nuclear magnetic resonance analysis of the degraded oligosaccharides, the backbone of such a polysaccharide mainly consisted of -> 4)-beta-D-GlcA-(1 -> 4)-alpha-L-Rha3S-(1 -> and -> 4)-beta-D-Xyl-(1 -> 4)-alpha-L-Rha3S-(1 -> disaccharide repeating units, and the ratio is approximately 4:1. In addition, about 4% of the xylose moieties bear sulfate groups. Minor amounts of branches containing hexose and unsaturated glucuronic acid were found during the sequence analysis of hexa- to octasaccharides. These results indicated the presence of a long branch in the ulvan. The clarification of the detailed structure provides a foundation for ulvan modification and its structure-activity relationship studies.
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