Structure and bioactivity of sulfated a-D-mannan from marine bacterium Halomonas halocynthiae KMM 1376T

Halomonas halocynthiae KMM 1376(T) is a Gram-negative bacterium that has been isolated from gill tissue of the ascidian Halocynthia aurantium. Mild acid hydrolysis of the lipopolysaccharide of H. halocynthiae KMM 1376(T) afforded an O-polysaccharide, which was studied by sugar analysis and NMR spectroscopy. The following structure of the O-polysaccharide presented as sulfated a-D-mannan was established: -> 2)-alpha-D-Manp3,6S-(1 -> 3)-alpha-D-Manp2Ac(similar to 71%)6S-(1 -> 3)-alpha-D-Manp-(1 ->. Study of biological activity has shown that sulfated alpha-D-mannan can specifically reduce the cell viability and colony formation of the human breast adenocarcinoma MDA-MB-231 cells in a concentration-dependent manner. In addition, polysaccharide inhibits epidermal growth factor induced neoplastic cell transformation in mouse epidermal JB6 Cl41 cells.