期刊
CARBOHYDRATE POLYMERS
卷 228, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.115359
关键词
Stichopus chloronotus; Fucosylated chondroitin sulfate; Chain conformation; Physicochemical properties; Gut microbiota; In vitro fermentation
资金
- National Natural Science Foundation of China [41806176]
- Shandong Provincial Natural Science Foundation [ZR2019PD006]
- Medicine and Health Science Technology Development Project of Shandong Province [2016WSB27019]
- Chinese Medicine Science Technology Development Project of Shandong Province [2017-206]
- Science and Technology Development Project of Weifang City [2019GX023]
This study investigated the chain conformation, physicochemical properties of fucosylated chondroitin sulfate from Stichopus chloronotus (fCS-Sc) using HPSEC-MALLS and worm-like cylinder model methods and its impact on human gut microbiota by 16s RNA gene sequencing. The results indicated that fCS-Sc adopted rigid rods chain conformation in PBS based on chain conformational parameters alpha(eta) (1.11) and alpha(h) (0.70). Stiffness parameters deduced from worm-like cylinder model suggested that fCS-Sc exhibited as stiff chains. The congo red assay indicated a single-helical structure. fCS-Sc demonstrated a negative zeta-potentials in a wide pH ranges and good thermal stability. Besides, fCS-Sc could modulate the community structure of gut microbiota by elevating the absolute abundance of microbiota, increasing the beneficial bacterium Megamonas, Bacteroides, Fusobacterium, Parabacteroides, Prevotella, Faecalibacterium, suppressing pathogenic microorganism and promoting the short-chain fatty acids production. The present studies improved the understanding of the spatial structures of fCSs and its application as prebiotics.
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