期刊
CANCER LETTERS
卷 477, 期 -, 页码 131-143出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2020.02.010
关键词
Single cell RNA sequencing; Nasopharyngeal carcinoma; Tumor microenvironment; Tumor heterogeneity; Tumor-infiltrating immune cells; T cell receptor
类别
资金
- National Natural Science Foundation of China [81972776, 81872278, 81772928, 81702907, 81672993, 81672683]
- Hunan provincial natural science fund [2018JJ3313, 2018JJ3815, 2018JJ3704, 2018SK21211, 2018SK21210]
- Hunan provincial health committee research project [C20180370]
- Sister Institution Network Fund of MD Anderson Cancer Center (2016)
- Natural Science Foundation of Guangdong Province, China (Guangdong Natural Science Foundation) [2018A0303100012]
- Key Laboratory of Translational Radiation Oncology [2015TP1009]
Nasopharyngeal carcinoma (NPC) is one of the most malignant tumors in Southern China and southeast Asia, which is characterized by a dense lymphocyte infiltration and a poor prognosis. The emergence of single-cell sequencing represents a powerful tool to resolve tumor heterogeneity and delineate the complex communication among the tumor cells with neighboring stromal and immune cells in the tumor microenvironment (TME). Here, we performed single cell RNA-seq and analyzed tumor cells together with the infiltrating immune cells from three NPC tumor tissues. In our study, the malignant cells display the intra- and inter-tumoral heterogeneity among the individual patients. Analysis of the immune cells reveal the heterogeneous composition of the distinct immune cells and the various functional states of T cells in NPC tumors. Additionally, coupled with the reconstruct of the T cell receptor (TCR) sequences from immune cells full-length single-cell sequence data, we identify the diverse T cell clonotypes and expansion distribution in individual tumors. Overall, we firstly reveal the landscape of tumor and infiltrating immune cells in nasopharyngeal cancer. These results provide deeper insights on the mechanisms of tumor clearance by immune cells in the surrounding microenvironment, which will be helpful in improving the targeted and immune therapies for NPC.
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