期刊
BIORESOURCE TECHNOLOGY
卷 196, 期 -, 页码 241-249出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.07.084
关键词
Lignocellulose bioconversion; Anaerobic consortium; Carboxylate production; Xylanase; Cellulase
资金
- French National Agency for Energy and Environment (ADEME)
- Carnot Institute 3BCAR
- INRA Metascreen project
A rumen-derived microbial consortium was enriched on raw wheat straw as sole carbon source in a sequential batch-reactor (SBR) process under strict mesophilic anaerobic conditions. After five cycles of enrichment the procedure enabled to select a stable and efficient lignocellulolytic microbial consortium, mainly constituted by members of Firmicutes and Bacteroidetes phyla. The enriched community, designed rumen-wheat straw-derived consortium (RWS) efficiently hydrolyzed lignocellulosic biomass, degrading 55.5% w/w of raw wheat straw over 15 days at 35 degrees C and accumulating carboxylates as main products. Cellulolytic and hemicellulolytic activities, mainly detected on the cell bound fraction, were produced in the earlier steps of degradation, their production being correlated with the maximal lignocellulose degradation rates. Overall, these results demonstrate the potential of RWS to convert unpretreated lignocellulosic substrates into useful chemicals. (C) 2015 Elsevier Ltd. All rights reserved.
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