期刊
BIORESOURCE TECHNOLOGY
卷 194, 期 -, 页码 334-343出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.06.144
关键词
Antrodia camphorata; Antrodin; Arthroconidium; Repeated batch fermentation
资金
- National Natural Science Foundation of China [31401931, 31201020]
- Industry-Academia Cooperation Innovation Fund Project of Jiangsu Province, China [BY2012052]
Large-scale submerged fermentation (SmF) of Antrodia camphorata (A. camphorata) usually encounters challenges including tedious preparation of mycelial inoculum, long fermentation period (10-14 d), and poor repeatability. Here we developed an asexual reproduction-based repeated batch fermentation (RBF) process for bioactive metabolites production by A. camphorata ATCC 200183. Compared with traditional batch fermentation, production time was shortened to 58 d from 80 d (overall time for eight cycles) using the RBF process established in this study, and accordingly, the productivities of bioactive metabolites (including antrodins) were improved by 40-60%. Kinetic parameters (alpha is 2.1-18.7 times as beta) indicated that the cell growth was the major contribution for bioactive metabolites production. The RBF shows excellent batch-repeatability (Pearson correlation coefficient of 0.998 +/- 0.001), together with advantages of energy-efficient, low cost, and labor-saving, RBF process can be implemented to SmF by other filamentous fungi. (C) 2015 Elsevier Ltd. All rights reserved.
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