期刊
BIORESOURCE TECHNOLOGY
卷 193, 期 -, 页码 288-296出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.06.096
关键词
Ambient-temperature sulfuric acid pretreatment; Water-soluble carbohydrates (WSCs); Hemicellulose adsorption; Cellulosic bio-ethanol; Alfalfa stems
资金
- National Institute of Food and Agriculture, United States Department of Agriculture [WIS01664]
- Wisconsin Energy Institute at the University of Wisconsin-Madison
Model compounds and recalcitrant biomass were studied to elucidate the mechanism of ambient-temperature acid pretreatment of cellulosic biomass for bio-ethanol production. Pure cellulose, a pure hemicellulose and alfalfa stems were pretreated with sulfuric acid under ambient temperature with varied acid loading and time. Changes in water-soluble carbohydrates (WSCs) and chemical components of substrates were determined, and ethanol production via simultaneous saccharification and fermentation (SSF) was studied. The results showed significant amount of WSCs formed, and the WSCs increased with increasing acid loading and pretreatment time. The ethanol yields from pure cellulose were primarily affected by the added ash. Acid loading showed significant positive effect on ethanol production from alfalfa stems, whereas pretreatment time showed much weaker positive effect. However, non-significant amounts of WSCs were removed by washing of dried substrates. It was hypothesized to be because the WSCs adsorbed onto bulk substrates during the freeze-drying step, as supported by experimental results. (C) 2015 Elsevier Ltd. All rights reserved.
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