期刊
BMC EVOLUTIONARY BIOLOGY
卷 20, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12862-019-1551-8
关键词
C-3; CAM; Carboxylation; Catalytic rate; Coevolution; Decision tree; Positive selection; Rubisco
资金
- FEDER/Spanish Ministry of Economy, Industry and Competitiveness -Research State Agency [AGL2009-07999, AGL2013-42364-R, PGC2018-096956-B-C43]
- Klaus-Tschira-Foundation gGmbH (Heidelberg, Germany)
- FPI Fellowship [BES-2010-030796]
Background The CO2-concentrating mechanism associated to Crassulacean acid metabolism (CAM) alters the catalytic context for Rubisco by increasing CO2 availability and provides an advantage in particular ecological conditions. We hypothesized about the existence of molecular changes linked to these particular adaptations in CAM Rubisco. We investigated molecular evolution of the Rubisco large (L-) subunit in 78 orchids and 144 bromeliads with C-3 and CAM photosynthetic pathways. The sequence analyses were complemented with measurements of Rubisco kinetics in some species with contrasting photosynthetic mechanism and differing in the L-subunit sequence. Results We identified potential positively selected sites and residues with signatures of co-adaptation. The implementation of a decision tree model related Rubisco specific variable sites to the leaf carbon isotopic composition of the species. Differences in the Rubisco catalytic traits found among C-3 orchids and between strong CAM and C-3 bromeliads suggested Rubisco had evolved in response to differing CO2 concentration. Conclusions The results revealed that the variability in the Rubisco L-subunit sequence in orchids and bromeliads is composed of coevolving sites under potential positive adaptive signal. The sequence variability was related to delta C-13 in orchids and bromeliads, however it could not be linked to the variability found in the kinetic properties of the studied species.
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