期刊
BMC BIOLOGY
卷 18, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s12915-020-0753-2
关键词
X-ray computed tomography; Correlative microscopy; Phase contrast; Lightsheet; Time-lapse tomography; 3D imaging; 3D histology; Elemental mapping; Quantitative tomography; Water window
类别
资金
- European Research Council (ERC) under the European Commission's Horizon 2020 (FP8/2014-2020) ERC grant [695638]
- Engineering and Physical Sciences Research Council (EPSRC) [EP/L014904/1]
- Medical Research Council [EP/L014904/1]
- Henry Royce Institute for Advanced Materials through EPSRC grants [EP/R00661X/1, EP/P025021/1, EP/P025498/1]
Recent developments within micro-computed tomography (mu CT) imaging have combined to extend our capacity to image tissue in three (3D) and four (4D) dimensions at micron and sub-micron spatial resolutions, opening the way for virtual histology, live cell imaging, subcellular imaging and correlative microscopy. Pivotal to this has been the development of methods to extend the contrast achievable for soft tissue. Herein, we review the new capabilities within the field of life sciences imaging, and consider how future developments in this field could further benefit the life sciences community.
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