期刊
BIOTECHNOLOGY LETTERS
卷 42, 期 3, 页码 419-428出版社
SPRINGER
DOI: 10.1007/s10529-019-02767-2
关键词
Hydrophobin; Dengue; Domain III; Rachiplusia nu larvae; ATPS
资金
- Universidad de Buenos Aires [UBACYT 2014-2017, No 298] Funding Source: Medline
- UBATEC [Primer Premio 2015] Funding Source: Medline
Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 mu g/larva of fusion protein DomIII serotype 1 and 61.4 mu g/larva of fusion protein DomIII serotype 2, represents an integrated strategy for simple production of recombinant antigens. Purified fusion proteins induced serotype-specific neutralizing antibodies without cross-reaction against other serotypes and arboviruses after mouse immunization. hydrophobin-fused domain III of dengue envelope protein could be a promising strategy for easy and low-cost production of components of a tetravalent sub-unit vaccine against dengue.
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