期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 117, 期 4, 页码 1187-1203出版社
WILEY
DOI: 10.1002/bit.27269
关键词
apoptosis; biopharmaceutical production; CHO cell line engineering; programmed cell death
资金
- Novo Nordisk Foundation [NNF10CC1016517, NNF14OC0009473]
Chinese hamster ovary (CHO) cells are the predominant host cell line for the production of biopharmaceuticals, a growing industry currently worth more than $188 billion USD in global sales. CHO cells undergo programmed cell death (apoptosis) following different stresses encountered in cell culture, such as substrate limitation, accumulation of toxic by-products, and mechanical shear, hindering production. Genetic engineering strategies to reduce apoptosis in CHO cells have been investigated with mixed results. In this review, a contemporary understanding of the real complexity of apoptotic mechanisms and signaling pathways is described; followed by an overview of antiapoptotic cell line engineering strategies tested so far in CHO cells.
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