期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 68, 期 1, 页码 20-29出版社
WILEY
DOI: 10.1002/bab.1886
关键词
acetylcholine esterase; biosensor; choline oxidase
Acetylcholine is a neurotransmitter associated with Alzheimer's disease due to its decrease in the central nervous system. A bienzymatic biosensor system was prepared to determine the amount of acetylcholine, utilizing enzymes and a modified carbon paste electrode. Factors affecting the biosensor response, such as temperature, pH, and substrate concentration, were investigated in this study.
Acetylcholine is a neurotransmitter, which is located at the intersections of the nerve and muscles in the lymph nodes of the internal organs motor systems and in various parts of the central nervous system. A decrease of acetylcholine in brain is associated with Alzheimer's disease. That is why it is an important agent for this disease. In this study, a bienzymatic biosensor system with acetylcholine esterase and choline oxidase was prepared with carbon paste electrode modified with carbon nano Dot-(3-Aminopropyl) triethoxysilane (CDs-APTES) for determination of the amount of acetylcholine. Acetylcholine esterase and choline oxidase enzymes were immobilized onto a modified carbon paste electrode by cross-linking with glutaraldehyde. Determination of acetylcholine was carried out by the oxidation of enzymatically produced H2O2 at 0.4 V versus Ag/AgCl. The effect of temperature, pH, and substrate concentration on the acetylcholine response of the prepared biosensor was investigated. In addition, the optimum CDs-APTES amount, the linear operating range of the biosensor, and the interference effect were also investigated.
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