期刊
BIOTECHNOLOGY ADVANCES
卷 40, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2019.107498
关键词
Assisted reproduction technologies; Lab-on-chip; Microfluidic devices; Non-invasive procedures; Human sperm; Animal sperm; Sperm selection; Sperm sorting; In vitro fertilization; ICSI
资金
- PON FSE - FESR 2014-2020 [CCI 2014IT16M2OP005, DOT1712250]
- Italian National FISR-CIPE Project InnoSense: Development of an innovative sensing platform for on-field analysis and monitoring (delibera CIPE) [78]
- European Project UE-H2020 - ICT project MADIA [732678]
- FEDER, through Programa Operacional Factores de Competitividade - COMPETE 2020
- FCT (Foundation for Science and Technology, Portugal) [POCI-01-0145-FEDER-007440, UID/NEU/04539/2019]
Male infertility often involves idiopathic or unknown causes, leading to an increasing demand for assisted reproduction technologies (ART). Conventional sperm sorting techniques rely on centrifugation steps that are known to cause oxidative stress and consequently damage cells. Alternative novel techniques have been introduced but offer disadvantages that need to be overcome. These techniques are also employed to increase the number and the quality of subjects in the animal breeding industry, to obtain purebred subjects or to preserve endangered animal species. Microfluidics deals with the manipulation of small amounts of volume within a microdevice known as lab-on-a-chip (LOC), which offers rapid analyses, ease of use, small reagent sample volumes, high-throughput processing and wide reproducibility owing to automation and standardization. As the LOC allows gamete handling within a microenvironment that strictly mimics physiological in vivo conditions and avoids centrifugation steps and long processing time, the use of microfluidics for sperm sorting and selection have been proposed during the last 15 years and is currently under investigation. Moreover, LOC technologies to sort, identify and analyse other kinds of cells could be transferred to sperm selection and analysis, thus opening the way to a novel approach to the sperm cell selection and manipulation. This review describes the techniques routinely performed in human and animal clinical practice for sorting good-quality sperm for in vitro fertilization procedures, and focuses on the positive and negative aspects of each method. Emerging microfluidic devices, recently proposed for sperm selection, are also described and, when possible, compared with standard methods.
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