期刊
BIOSENSORS & BIOELECTRONICS
卷 156, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112109
关键词
2D material; Antibody; Biosensor; Electrochemistry; Graphene oxide
类别
资金
- EU Graphene Flagship Core 2 Project [785219]
- CERCA Program/Generalitat de Catalunya
- Spanish MINECO [MAT2017-87202-P]
- Severo Ochoa Centers of Excellence Program [SEV2201320295]
- CEITEC 2020 [LQ1601]
- Grant Agency of the Czech Republic [GACR 20-30129Y]
- Ministry of Education, Youth and Sports of the Czech Republic under the CIISB research infrastructure project [LM2015043]
The design and application of an inkjet-printed electrochemically reduced graphene oxide microelectrode for HT-2 mycotoxin immunoenzymatic biosensing is reported. A water-based graphene oxide ink was first formulated and single-drop line working microelectrodes were inkjet-printed onto poly(ethylene 2,6-naphthalate) substrates, with dimensions of 78 mu m in width and 30 nm in height after solvent evaporation. The printed graphene oxide microelectmdes were electrochemically reduced and characterized by Raman and X-ray photoelectron spectroscopies in addition to microscopies. Through optimization of the electrochemical reduction parameters, differential pulse voltammetry were performed to examine the sensing of 1-naphthol (1-N), where it was revealed that reduction times had significant effects on electrode performance. The developed microelectrodes were then used as an immunoenzymatic biosensor for the detection of HT-2 mycotoxin based on carbodiimide linking of the microelectmde surface and HT-2 toxin antigen binding fragment of antibody (anti-HT2 (10) Fab). The HT-2 toxin and anti-HT2 (10) Fab reaction was reported by anti-HT2 immune complex single-chain variable fragment of antibody fused with alkaline phosphatase (anti-IC-HT2 scFv-ALP) which is able to produce an electmactive reporter - 1-N. The biosensor showed detection limit of 1.6 ng . mL(-1) and a linear dynamic range of 6.3 - 100.0 ng . mL(-1) within a 5 min incubation with 1-naphthyl phosphate (1-NP) substrate.
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