期刊
BIOSENSORS & BIOELECTRONICS
卷 146, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111746
关键词
Circulating tumor cells; Cancer detection; Conducting polymer; Amperometric separation detection; Electrochemical microfluidic device
类别
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2019R1A2C1002531]
- National Research Foundation of Korea [2019R1A2C1002531] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Different circulating tumor cells (CTCs) in blood were separated and detected through the decoration of anticancer drug on the target cells, along with chemical modification of the microfluidic channel walls using a lipid attached covalently to the conducting polymer. The working principle of the electrochemical microfluidic device was evaluated with experimental parameters affecting on the separation, in terms of mass and surface charge of target species, fluid flow rate, AC amplitude, and AC frequency. The separated CTCs were selectively detected via the oxidation of daunomycin adsorbed specifically at the cells using an electrochemical sensor installed at the channel end. The fluorescence microscopic examination also confirmed the separation of CTCs in the channel. To evaluate the reliability of the method, blood samples from 37 cancer patients were tested. The device was able to separate the CTCs with 92.0 +/- 0.5 % efficiency and 90.9% detection rate.
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