期刊
BIOSENSORS & BIOELECTRONICS
卷 148, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111666
关键词
Zn2+; Fluorescence imaging; Ratiometric; Two-photon; Neurons; Biosensors
类别
资金
- National Natural Science Foundation of China (NSFC) [21635003]
- NSFC for distinguished Young Scholars [21325521]
- Program of Shanghai Subject Chief Scientist [15XD1501600]
- Innovation Program of Shanghai Municipal Education Commission [201701070005E00020]
As an essential transition-metal ion in organism, zinc ion (Zn2+) plays a significant role in cellular metabolism, apoptosis and neurotransmission. Herein, we reported a new intramolecular charge transfer-based two-photon probe, (E)-1-(6-(4-(2-(2-(2-methoxyethoxy)ethoxy)eth-oxy)-3,5-dimethylstyryl)quinolin-2-yl)-N,N-bis(pyridin-2-ylmethyl)methanamine (TEO-MPVQ), for ratiometric imaging and biosensing of Zn2+ in living cells and larval zebrafish. The maximum two-photon absorption cross-section values (delta(max)) of the developed probe increased from 115 +/- 16 GM to 188 +/- 27 GM, after the probe was chelated with Zn2+. The emission spectrum of TEO-MPVQ probe increased and red-shifted about 60 nm, resulting in the ratiometric determination of Zn2+ with high sensitivity and selectivity. The present fluorescent probe also demonstrated good biocompatibility, high spatial resolution and deep penetration depth for Zn2+ detection. Accordingly, TEO-MPVQ was able to track the endogenous Zn2+ release in neurons and also successfully applied for larval zebrafish imaging. By using of this effective probe, it was found that the endogenous Zn2+ was rapidly released from metallothionein under the stimulation of NO in neurons.
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