期刊
BIOSENSORS & BIOELECTRONICS
卷 150, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.111925
关键词
Electrochemical magneto-immunosensor; Paper microfluidic electrode; Malaria quantitative diagnosis; Plasmodium LDH; Point-of-care (POC) testing
类别
资金
- Fondo de Investigaciones Sanitarias of the Institute de Salud Carlos III, ISCIII
- European Regional Development Fund, ERDF)
- Generalitat de Catalunya [2017-SGR-908]
- VHIR - Amics del VHIR
- INPhINIT fellowship from La Caixa Foundation [100010434, LCF/BQ/DI18/11660061]
- European Union [713673]
- PERIS grant from the Departament de Salut of the Generalitat de Catalunya (Spain) [SLT002/16/00316]
- Juan Rocks postdoctoral fellowship from ISCIII [JR18/00022]
- European Commission under Horizon 2020's Marie Sklodowska-Curie Actions COFUND scheme [712754]
- Spanish Ministry of Science and Competitiveness [SEV-2014-0425]
- Secretaria d'Universitats i Recerca del Departament d'Empresa i Coneixement, Generalitat de Catalunya [2017 SGR 240]
- Fundacion Ramon Areces
- Ministerio de Ciencia, Innovation y Universidades, Spain
- ERDF
- [CPII18/00025]
- [DTS17/00145]
- [RTI2018-094579-B-I00]
Malaria, a parasitic infection caused by Plasmodium parasites and transmitted through the bite of infected female Anopheles mosquitos, is one of the main causes of mortality in many developing countries. Over 200 million new infections and nearly half a million deaths are reported each year, and more than three billion people are at risk of acquiring malaria worldwide. Nevertheless, most malaria cases could be cured if detected early. Malaria eradication is a top priority of the World Health Organisation. However, achieving this goal will require mass population screening and treatment, which will be hard to accomplish with current diagnostic tools. We report an electrochemical point-of-care device for the fast, simple and quantitative detection of Plasmodium falciparum lactate dehydrogenase (PfLDH) in whole blood samples. Sample analysis includes 5-min lysis to release intracellular parasites, and stirring for 5 more min with immuno-modified magnetic beads (MB) along with an immuno-modified signal amplifier. The rest of the magneto-immunoassay, including sample filtration, MB washing and electrochemical detection, is performed at a disposable paper electrode microfluidic device. The sensor provides PfLDH quantitation down to 2.47 ng mL(-1) in spiked samples and for 0.006-1.5% parasitemias in Plasmodium-infected cultured red blood cells, and discrimination between healthy individuals and malaria patients presenting parasitemias >0.3%. Quantitative malaria diagnosis is attained with little user intervention, which is not achieved by other diagnostic methods.
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