期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 84, 期 5, 页码 919-926出版社
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2020.1714420
关键词
Alzheimer's disease; snRNA-seq; APP23 mouse model; hippocampal neurons
类别
资金
- Shanghai Science and Technology Development foundation [16JC1420502]
- National Natural Science Foundation of China [31800855, 31530089]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDPB10]
- Fundamental Research Funds for the Central Universities
Alzheimer's disease (AD) is a progressive neurodegenerative disorder that mostly strikes the elderly. However, the exact molecular and cellular pathogenesis of AD, especially the dynamic changes of neurons during disease progression, remains poorly understood. Here we used single-nucleus RNA sequencing (snRNA-seq) to access the transcriptional changes of hippocampal neurons in APP23 mouse model of AD. We performed snRNA-seq using a modified Smart-seq2 technique on 3,280 neuronal nuclei from the hippocampus of young and aged APP23 and control mice and identified four distinct subpopulations. Comparative transcriptional analysis showed multiple changes in different subtypes of hippocampal neurons of APP23 mice in comparison to control mice, as well as the transcriptional changes in these neurons during disease progression. Our findings revealed multiple neuronal subtype-specific transcriptional changes that may lead to targets for future studies of AD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据