期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 84, 期 3, 页码 544-551出版社
OXFORD UNIV PRESS
DOI: 10.1080/09168451.2019.1697197
关键词
Genistein; lipopolysaccharide; mouse lung epithelial (MLE)-12; alveolar epithelial
类别
资金
- 2019 Zhejiang Health Department General Project [2019319359]
Alveolar and bronchial epithelial cells have critical functions in acute respiratory distress syndrome progress. Genistein could protect the lungs from acute lung injury, however, whether genistein protects the alveolar epithelial cells from LPS-induced injury was less studied. Spectrophotometric method 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and enzyme-linked immunosorbent assay (ELISA) were performed to detect cell viability and levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), and IL-6. Flow cytometry and western blot assay were performed to detect cells apoptosis and protein levels. In LPS-induced model of mouse lung epithelial (MLE)-12 cells, PBEF (proinflammatory cytokine) expression, and cell apoptosis were increased and cell viability was decreased, whereas NF-kappa B was activated and expression levels of TNF-alpha, IL-1 beta, and IL-6 were increased. However, genistein partly reversed the effect of LPS, and it plays a protective role in lung injury by reducing expression of PBEF, inhibiting the activation of NF-kappa B and alleviating inflammatory response of cells.
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