Enhanced butanol-hydrogen coproduction by Clostridium beijerinckii with biochar as cell's carrier

In this study, the effects of biochar on the fermentation performance of butanol-hydrogen coproduction by Clostridium beijerinckii F-6 were investigated. Results showed that the biochar with rich porous and graphitized structure can significantly promote the coproduction of butanol and hydrogen. The productivity of butanol and hydrogen reached 0.148 g/L/h and 0.299 mmol/L/h with biochar addition which were 20.23% and 48.76% higher than that in control without biochar addition, respectively. Moreover, the whole energy conversion efficiency calculated based on the heat value showed increment from 43.69% to 51.75% with biochar addition. Combined analysis of organic acids accumulation and oxidation-reduction potential fluctuation proved that biochar can regulate reducing power during fermentation and accelerate the conversion of acid phase to solvent phase. Scanning electron microscope images showed that biochar acted as carriers for cells absorption. Confirmation experiment further proved that biochar enhanced the butanol tolerant ability of Clostridium beijerinckii F-6.