Nitrogen removal mechanism and microbial community changes of bioaugmentation subsurface wastewater infiltration system

Limited nitrogen removal capacity (mainly nitrate, NO3--N) remains a major challenge for subsurface wastewater infiltration system (SWIS). Two nitrogen-removing strains have been isolated from SWIS and inoculated to SWIS to investigate the effect of bioaugmentation on nitrogen removal performance and mechanism. The results showed bioaugmentation improved the removal efficiencies of NH4+-N from 86.81% to 92.86% and TN from 74.90% to 86.55% and running stability compared to unbioaugmentation SWIS. 16 s rRNA amplicon sequencing results of the bacterial indicated that bioaugmentation altered the microbial community structure especially at 150 cm depth and increased the relative abundance of bacteria associated with nitrogen removal, significantly increasing the abundance of Rhizobiales_Incertae_Sedis and Lachnospiraceae. Furthermore, the relation between internal microbial characteristics and operational factors indicated that Hyphomicrobiaceae and Gemmatimonadaceae were also closely related to nitrogen removal. Predicted function profiles revealed that bioaugmentation enhanced the activity of nitrogen removal enzymes (Hao, NorBC, NasAB, NarGHI, NirBD and NosZ).