期刊
BIORESOURCE TECHNOLOGY
卷 298, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122534
关键词
Anaerobic digestion; Direct interspecies electron transfer (DIET); Community analysis; 16S RNA analysis; Low strength wastewater
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- NSERC Industrial Research Chair (IRC) Program in Sustainable Urban Water Development
- EPCOR Water Services
- EPCOR Drainage Operation
- Alberta Innovates
- WaterWerx
- Canada Research Chair (CRC) in Future Community Water Services
Granular activated carbon (GAC) has been shown to mediate direct interspecies electron transfer (DIET) in anaerobic digestion. Adding GAC to up-flow anaerobic sludge bed reactor increased the total biomass slightly from 20.0 to 26.6 gVSS/reactor, and maximum organic removal capacity remarkably from 285 to 1660 mgCOD/L/d. Since GAC occupied 7% of reactor volume (denser than suspended sludge, settled to the reactor bottom), we used a spatial sampling strategy (sludge bed top/mid/bottom layers, and tightly attached GAC-biofilm) and DNA- and RNA-based community analyses. RNA-based analysis demonstrated significant community differences between the non-GAC and GAC-amended reactors (p < 0.05) based on ANOSIM statistical analysis. In comparison, DNA-based analysis showed little community difference between these reactors (p > 0.05). RNA-based analysis revealed active enrichments in GAC-biofilm, including bacteria Geobacter, Syntrophus, Desulfovibrio and Blvii28, and archaea Methanosaeta and Methanospirillum. These are potential electro-active syntrophic microorganisms related with DIET, which expand the previously defined list of DIET microorganisms.
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