期刊
BIORESOURCE TECHNOLOGY
卷 296, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122285
关键词
Miscanthus; Steam explosion; Xylo-oligosaccharides; Biofuels; Biorefining
资金
- Biotechnology and Biological Sciences Research Council (BBSRC)
- Sao Paulo Research Foundation (FAPESP) [BB/P017460/1]
- BBSRC [BB/CSP1730/1, BB/N016149/1, BB/K01711X/1, BBS/E/W/10963A01]
- GIANT-LINK project [LK0863]
- BEACON Biorefining Centre of Excellence
- BBSRC [BB/N016149/1, BB/P017460/1, BBS/E/W/10963A01D, BB/R000700/1, BBS/E/W/0012843A] Funding Source: UKRI
This study investigated pilot-scale production of xylo-oligosaccharides (XOS) and fermentable sugars from Miscanthus using steam explosion (SE) pretreatment. SE conditions (200 degrees C; 15 bar; 10 min) led to XOS yields up to 52 % (w/w of initial xylan) in the hydrolysate. Liquid chromatography-mass spectrometry demonstrated that the solubilised XOS contained bound acetyl- and hydroxycinnamate residues, physicochemical properties known for high prebiotic effects and anti-oxidant activity in nutraceutical foods. Enzymatic hydrolysis of XOS-rich hydrolysate with commercial endo-xylanases resulted in xylobiose yields of 380 to 500 g/kg of initial xylan in the biomass after only 4 h, equivalent to similar to 74 to 90 % conversion of XOS into xylobiose. Fermentable glucose yields from enzymatic hydrolysis of solid residues were 8 to 9-fold higher than for untreated material. In view of an integrated biorefinery, we demonstrate the potential for efficient utilisation of Miscanthus for the production of renewable sources, including biochemicals and biofuels.
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