期刊
BIOMEDICAL CHROMATOGRAPHY
卷 34, 期 5, 页码 -出版社
WILEY
DOI: 10.1002/bmc.4805
关键词
HPLC-Q-TOF; intestinal bacteria; liver; metabolism; Qushi Huayu Fang
类别
资金
- Youth Innovation Promotion Association of Chinese Academy of Sciences [2019280]
- Shanghai Science and Technology Development Funds [1840193310, 18401931300]
- National Science and Technology Major Project of the Ministry of Science and Technology of China [2019ZX09201001-001-002, 2019ZX09201001-001-008]
- National Natural Science Foundation of China [81973456]
Qushi Huayu Fang (QHF) is a clinic-empirical prescription for treating non-alcoholic fatty liver disease (NAFLD) in China, which is composed of five herbs. However, the bioactive constituents responsible for the efficacy of QHF remain unclear. Thus, a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method was established and adopted to identify the constituents of QHF, and profile its metabolism in vivo and in vitro. Among the 66 constituents in QHF, only 14 compounds of six structural types were absorbed, and 34 metabolites were generated through eight metabolic pathways. A total of 20 metabolites were first reported, including four organic acids, one iridoid, two flavones, five naphthols, three anthraquinones, and five stilbenes. Glucuronidation and sulfation were the main metabolic pathways, and the intestinal metabolism played an important role in the biotransformation of QHF. Many compounds, especially those detected in the liver, the target organ of QHF, were reported to display the anti-NAFLD activity. This is the first study to explore the constituents of QHF and its metabolism in vivo and in vitro, thus realizing the first step to clarify the chemical basis of QHF qualitatively, and laying the foundation for further research on the anti-NAFLD mechanism of QHF.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据