期刊
BIOFOULING
卷 36, 期 1, 页码 101-112出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2020.1719077
关键词
Foodborne pathogens; cross-resistance; Salmonella Enteritidis; chemical disinfectants; biofilm; virulence
资金
- Portuguese Foundation for Science and Technology (FCT) [UIDB/04469/2020]
- BioTecNorte operation - European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000004]
Although disinfection procedures are widely implemented in food environments, bacteria can survive and present increased virulence/resistance. Since little is known about these phenomena regarding biofilms, this study aimed to investigate the effect of chemical disinfection on biofilm-derived cells of Salmonella Enteritidis. Using a reference strain (NCTC 13349) and a food isolate (350), biofilm susceptibility to benzalkonium chloride (BAC), sodium hypochlorite (SH) and hydrogen peroxide (HP) was evaluated and biofilms were exposed to sub-lethal concentrations of each disinfectant. Biofilm-derived cells were characterized for their biofilm forming ability, antibiotic resistance and expression of virulence-associated genes. Except for a few instances, disinfectant exposure did not alter antibiotic susceptibility. However, SH and HP exposure enhanced the biofilm forming ability of Salmonella Enteritidis NCTC 13349. After BAC and HP exposure, biofilm-derived cells presented a down-regulation of rpoS. Exposure to BAC also revealed an up-regulation of invA, avrA and csgD on Salmonella Enteritidis NCTC 13349. The results obtained suggest that biofilm-derived cells that survive disinfection may represent an increased health risk.
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