期刊
AQUATIC TOXICOLOGY
卷 218, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.aquatox.2019.105362
关键词
Nitrite; Immune response; Inflammatory cytokines; Takifugu rubripes
资金
- National Key R&D Program of China [2017YFD0701701]
- Central Public-interest Scientific Institution Basal Research Fund, YSFRI, CAFS [20603022018015]
- Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, the Ministry of Agriculture and Rural Affairs, Dalian Ocean University, the P. R. China [2018-KF-02]
- Modern Agriculture Industry System Construction of Special Funds [CARS-47]
- Demonstration project of collaborative innovation in the industry chain of intelligent cage assembly equipment of far-reaching sea
Nitrite is a major environmental pollutant in aquatic environments that negatively affects aquatic species. In this study, we investigated the impact of nitrite exposure on plasma biochemical parameters and immune responses in Takifugu rubripes. Fish were exposed to various concentrations of nitrite (0, 0.5, 1, 3, and 6 mM) for 96 h. After 0, 12, 24, 48, and 96 h of exposure, fish blood samples were collected to assay the levels of total protein (TP), albumin (Alb), glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (ALT), complement C3 (C3), complement C4 (C4), immunoglobulin (IgM), and lysozyme activity (LZM). The gills were sampled to analyze the mRNA levels of heat shock protein 70 (hsp70), heat shock protein 90 (hsp90), tumor necrosis factor alpha (tnf-alpha), B-cell activating factor (baff), interleukin-6 (il-6), and interleukin-12 (il-12). Levels of GOT, ALT, C3, and C4 were significantly enhanced in the high nitrite concentration group (3 and 6 mM), whereas those of TP, Alb, LZM, and IgM decreased significantly with the same treatments. Nitrite significantly upregulated hsp70, hsp90, tnf-alpha, il-6, il-12, and baff mRNA levels after 96 h of exposure. These results indicated that nitrite exposure altered the blood physiological status and immune system response, resulting in dysfunction and immunotoxicity in T. rubripes. Furthermore, our results reveal the possible mechanism of aquatic-nitrite-induced toxicity in fish.
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